Project Details:

Reference methods, standards and applications of photoluminescence

Project No.:2004-021-1-300
Start date:2004-07-01
End date:2010-11-02
Division:Physical and Biophysical Chemistry Division
Objective:

IUPAC documents on fluorescence will be updated including advances registered during the last fifteen years. Particular attention will be given to newly developed reference materials and methods.

Description:

Fifteen years ago, David Eaton undertook the task of collecting information on fluorescence methods and materials as a task of the IUPAC Commission on Photochemistry. Two relevant documents evolved from this work: 1) Reference Materials for Fluorescence Measurement, Pure Appl. Chem., 60(7), 1107-1114 (1988); 2) Recommended Methods for Fluorescence Decay Analysis, Pure Appl. Chem., 62(8), 1631-1648 (1990).

Since Eaton's documents were written, new reference materials appeared and the subject evolved rapidly with the incorporation of new areas and methods which were not or only poorly developed at that time. A few examples are single molecule fluorescence, ultrafast fluorescence detection, fluorescence microscopy and so forth. Many of these areas are of utmost relevance in materials science and in biology. In general, the interest shifted impressively to organized, (micro)heterogeneous systems.

Through the present project we propose an update of Eaton's documents, with a renewed body of reference information and the incorporation of a brief overview on new methods and techniques which may guide those entering the field through this fascinating topic. At the same time, the scope of the work will be broadened to include luminescence from molecular emitters in their triplet state or states with mixed spin multiplicity, quantum dots, etc. Thus, the term "fluorescence" appearing in the earlier documents has been replaced by the far more comprehensive term "photoluminescence". Reference methods will include recommendations for the calibration of luminescence measuring systems, the correction of emission, excitation, action and polarization spectra, the determination of quantum yields in different spectral ranges and the determination of lifetimes or decay kinetics in the various achievable time scales. Whenever possible, extension to non-dilute, unclear or complex systems of applied interest will be performed.

On defining the task group people were selected, both who participated in the earlier projects, giving continuity to the work already done, and new scientists. Together they will constitute a team having a broad and active view on the subject.

Progress:

The Task Group joined at the XX IUPAC Symposium on Photochemistry, held in July 2004 in Granada, and yearly since. It was decided, taking into account the high number of participants, that the project would be divided into subtasks. Up to now, a provisional subdivision has been achieved (see report below)
> Nov 2004 report update (pdf file - 17KB)

> Project annoucement publsihed in Chem. Int. Nov-Dec 2004

> May 2006 report update (pdf file - 13KB)

> July 2007 report update (pdf file - 10KB)

The project is divided into different sections:
I. Steady-state Luminescence Measurements
II. Time-resolved Spectroscopy and Decay Analysis
III. Single Molecules and Microfluorimetry
IV. Luminescence Measurement in Microheterogeneous, Heterogeneous, highly Absorbing and Complex System

> August 2009 - Nearly 15 documents will be prepared as outcome of this project. The first titles in the series are:
1. Fluorescence Standards: Classification, Terminology and Recommendations on their Selection, Use and Production, by U. Resch-Genger, P. C. DeRose
2. Characterization of Photoluminescence Measuring Systems, by U. Resch-Genger, P. C. DeRose
3. Determination of the Photoluminescence Quantum Yield of Dilute Dye Solutions, by K. Rurack, U. Resch-Genger
4. Standards for Photoluminescence Quantum Yield Measurements in Solution, by A. M. Brouwer
5. Fluorescence Anisotropy Measurements in Solution: Methods and Reference Materials, by M. Ameloot, M. vandeVen, A. U. Acuña , B. Valeur
6. Time-Resolved Fluorescence Methods, by H. Lemmetyinen, N. Tkachenko, B. Valeur, N. Boens, M. Ameloot, N. Ernsting, T. Gustavsson, J.-I. Hotta

These documents will require formal acceptance by IUPAC through its internal bodies and external reviewers. Opinions, criticisms and comments from the photochemical community are kindly requested; to request a draft for preview, please contact the task group chair Enrique San Román, by email at esr@qi.fcen.uba.ar.

> project update report published in Chem. Int. Nov-Dec 2009

 Project Completed - technical report published in Pure Appl. Chem., 2010, Vol. 82, No. 12, pp. 2315-2335 (doi:10.1351/PAC-REP-09-09-02)

> Aug 2011 - a second report titled 'Standards for photoluminescence quantum yield measurements in solution (IUPAC Technical Report)' has been published, doi:10.1351/PAC-REP-10-09-31 (online 2011-08-31)

Reference: Pure Appl. Chem., 2011, Vol. 83, No. 12, pp. 2213-2228

> Aug 2012 - an additional report “Characterization of photoluminescence measuring systems” has been completed and published as Pure Appl. Chem., 2012, Vol. 84, No. 8, pp. 1815-1835; http://dx.doi.org/10.1351/PAC-REP-10-07-07

> Mar 2013 - an additional report “Fluorescence anisotropy measurements in solution: Methods and reference materials (IUPAC Technical Report)“ has been completed and published as Pure Appl. Chem., 2013, Vol. 85, No. 3, pp. 589-608; http://dx.doi.org/10.1351/PAC-REP-11-11-12

> May 2013 - an additional report “Fluorescence correlation spectroscopy (IUPAC Technical Report)“ has been completed and published as Pure Appl. Chem., 2013, Vol. 85, No. 5, pp. 999-1016; http://dx.doi.org/10.1351/PAC-REP-11-11-17

 

Last update 7 June 2013

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