Project Details:

Direct derivatization of organic compounds at trace level concentrations for fluorescence detection and capillary electrophoresis Series: Analytical Techniques for Trace Organic Compounds

Derivatization of trace organic compounds to enhance the separation of trace compounds from the matrix or from other interfering components has been developed for several chromatographic techniques. Further, well chosen derivatives can enhance the detection capabilities of a given system considerably. Even though HPLC is generally considered a technique which needs no derivatization, under special circumstances such a step can be great advantage, particularly to put detectors with superior detection capabilities to use or to allow a change of phases such as from straight phase to reversed phase. For trace analysis of real world samples in difficult matrices this is a relevant option and needs consideration. In this project the possibilities, advantages and disadvantages shall be discussed in such a critical manner that the reader can consider to use this option avoiding the already known pitfalls.