Project Details:

Hold-up volume in column chromatography

Although it is a fundamental concept in chromatography the hold-up or void volume) of a column system has been defined in different ways in different systems. A greater difficulty is lack of agreement on the methods that can be used to determine the measurement. The aim of the project is to provide a critical review of the background and theory of the meaning of the terminology and to critically compare the different methods of measurement and of the significance of the values that they generate.

• A final draft has circulated to Commission members and submitted tothe Division. Some of the terms have been coordinated with project530/13/97.
• A revised document is submitted to public review comments until 28February 2001
  > see provisionalrecommendations
• A combined manuscript entitled 'Retention Parameters in Chromatography.Part A. The Hold-Up Volume Concept in Column Chromatography. Part B.Retention Parameters in Gas Chromatography.' is published in PureAppl. Chem.73(6), pp. 969-992, 2001