Pure Appl. Chem., 1999, Vol. 71, No. 2, pp. 349-359
doi:10.1351/pac199971020349
CHEMISTRY AND THE ENVIRONMENT DIVISION
COMMISSION ON OILS, FATS AND DERIVATIVES
The determination of stigmastadienes in vegetable oils. Results of collaborative studies and the standardized method
M.C. Dobarganes, A. Cert and A. Dieffenbacher
Abstract:
The development by collaborative study of a standardised method for the determination of the stigmastadienes content in vegetable oils is described. The procedure involves the isolation of the unsaponifiable matter in the oil ands its fractioning on a silica gel column. The fraction containing the steroidal hydrocarbons is then analysed by capillary gas chromatography and the quantitative assessment made by the use of an internal standard /cholea-3,5-staddiene). The procedure is very sensitive allowing determination at levels of 0.01 - 4.0 mg/kg.