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Pure Appl. Chem., 1999, Vol. 71, No. 10, pp. 1983-1991

http://dx.doi.org/10.1351/pac199971101983

CHEMISTRY AND THE ENVIRONMENT DIVISION
COMMISSION ON OILS, FATS AND DERIVATIVES

Determination of mono- and diacylglycerols in edible oils and fats by high performance liquid chromatography and evaporative light scattering detector: Results of collaborative studies and the standardised method

D. Berner and A. Dieffenbacher

Abstract: The development, by collaborative study, of standardised method for the determination of mono- and diacylglycerols in vegetable oils and fats is described. The method involves separation of mono- and diacylglycerols by normal phase high-performance liquid-liquid chromatography (HPLC) and evaporative light scattering detection of a solution of oil, fat or a commercial mono- and diacylglycerol preparation in a organic solvent.