Production of a novel alkaline lipase by <i>Fusarium globulosum</i> using neem oil, and its applications

Gulati, Ruchi; Isar, Jasmine; Kumar, Vineet; Prasad, Ashok K.; Parmar, Virinder S.; Saxena, Rajendra K.

doi:10.1351/pac200577010251

Pure Appl. Chem., 2005, Vol. 77, No. 1, pp. 251-262

http://dx.doi.org/10.1351/pac200577010251

Production of a novel alkaline lipase by Fusarium globulosum using neem oil, and its applications

Ruchi Gulati¹, Jasmine Isar¹, Vineet Kumar², Ashok K. Prasad², Virinder S. Parmar² and Rajendra K. Saxena¹

¹ Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi 110 021, India
² Bioorganic Laboratory, Department of Chemistry, University of Delhi, Delhi 110 007, India

Abstract: A low-temperature, alkaline, and detergent-compatible lipase from Fusarium globulosum (FGL) has been produced using an inexpensive source, viz. neem oil. Maximum lipase production of 12 300 U/L was achieved in 96 h on optimizing various physicochemical parameters. The lipase exhibited remarkable stability in the presence of commercial detergents, bleaching agents, and proteases. The lipase showed a novel property of marked activation on prolonged incubation at alkaline pH. Wash performance analysis of the commercial detergent for removal of fatty stains improved upon addition of this lipase.